BDNF Antibody (FITC)

SKU: orb377478

Description

Rabbit polyclonal antibody against BDNF conjugated to FITC.

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution of SPC-703 was sufficient for detection of BDNF on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal of human BDNF
• Target: BDNF
• Molecular Weight: 27.9kDa
• Purification: Peptide Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

BDNF, BDNF_HUMAN, Brain-derived neurotrophic factor, Abrineurin, ANON2, MGC34632, Neuronal differentiation factor

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Lane 3: Human 293T (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.

Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.

Quick Database Links

Gene Symbol

BDNF

UniProt

P23560

NCBI Gene ID

627

RefSeq (Protein):NP_001137277.1