DEFB105A Antibody (Biotin)

SKU: orb1671416

Description

DEFB105A Antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human BD-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5. Western Blot To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography and then biotinylated.
• Target: DEFB105A
• Purification: BD-5 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BD5, DEFB5, DEFB105a, Beta-Defensin 5

To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BD-5 as a capture antibody, allows them detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5.

To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

DEFB105A

UniProt

Q8NG35

RefSeq:Q8NG35