You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
BD-5 Antibody (biotin)
Catalog Number: orb1671416
| Catalog Number | orb1671416 |
|---|---|
| Category | Antibodies |
| Description | BD-5 Antibody (biotin) |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography and then biotinylated. |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| Target | DEFB105A |
| UniProt ID | Q8NG35 |
| NCBI | Q8NG35 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | BD5, DEFB5, DEFB105a, Beta-Defensin 5 Read more... |
| Note | For research use only |
| Application notes | Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human BD-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5. Western Blot To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BD-5 as a capture antibody, allows them detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5.
To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
