Bcl-x (phospho Thr47) rabbit pAb

• Catalog Number: orb769910
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Bcl-x (phospho-Thr47)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human BCL-XL around the phosphorylation site of Thr47. AA range:13-62
• MW: 30
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-BCL2L1 antibody, anti-BCL2L antibody, anti-BC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAT-MUSLE using Phospho-Bcl-x (T47) antibody. Antibody was diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BCL-XL (Phospho-Thr47) Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.