Bcl-X Antibody

SKU: orb2643750

Description

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Bcl-X antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: BCL2L1/2406
• Immunogen: A recombinant human partial protein (amino acids 1-135) was used as the immunogen for this Bcl-X antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human Hodgkin's lymphoma with Bcl-X antibody (clone BCL2L1/2406). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hodgkin's lymphoma with Bcl-X antibody (clone BCL2L1/2406). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Bcl-X antibody (clone BCL2L1/2406) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q07817