Bcl-w rabbit pAb

• Catalog Number: orb764635
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Bcl-w
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human BCLW. AA range:131-180
• MW: 25
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-BCL2L2 antibody, anti-BCLW antibody, anti-KIA
• Note: For research use only

Western Blot analysis of various cells using Bcl-w Polyclonal Antibody diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HepG2 cells, using BCLW Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using BCLW Antibody. The lane on the right is blocked with the synthesized peptide.