BCKDHB Antibody

• Catalog Number: orb1270678
• Category: Antibodies
• Description: BCKDHB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: BCKDHB
• UniProt ID: P21953
• NCBI: P21953
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: 2-oxoisovalerate dehydrogenase subunit beta, mitoc
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in Hela cell line lysates (35 ug/lane).

BCKDHB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of BCKDHB using rabbit polyclonal BCKDHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of BCKDHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).