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BCAP31 Antibody

SKU: orb1248011

Description

BCAP31 Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Placenta and Brain lysate (predicted MW of 28kDa according to NP_005736). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney and Brain (Cerebellum and Cortex). Recommended concentration: 3.75ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-QAAVDGPMDKKEE
TargetBCAP31
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

BCAP31, BAP31, DXS1357E, accessory protein BAP31, CDM, 6C6-AG, B-cell receptor-associated protein 31, CDM, 6C6 antigen, BCR-associated protein Bap31, OTTHUMP00000025978, p28 Bap31

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BCAP31 Antibody

orb1248011 (0.1 ug/ml) staining of Human Breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BCAP31 Antibody

orb1248011 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

BCAP31 Antibody

orb1248011 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

BCAP31 Antibody

orb1248011 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

BCAP31 Antibody (orb1248011)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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