KRT76 Antibody

SKU: orb1252385

Description

KRT76 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Application Notes:
  ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: KRTH/1076
• Immunogen: Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.
• Target: KRT76
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, CK-2P, HUMCYT2A

Formalin paraffin human skin stained with Basic Cytokeratin antibody (KRTH/1076).

Formalin paraffin rat stomach stained with Basic Cytokeratin antibody (KRTH/1076).

Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).

SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone KRTH/1076). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

KRT76

UniProt

Q01546