BAP1 Antibody / BRCA1-Associated Protein 1

SKU: orb606862

Description

The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the BAP1 antibody to be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c, kappa
• Clone No.: BAP1/2431
• Immunogen: A portion of amino acids 191-326 from the human protein was used as the immunogen for the BAP1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using BAP1 antibody (clone BAP1/2431). These results demonstrate the foremost specificity of the BAP1/2431 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free BAP1 antibody (clone BAP1/2431) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q92560