BAG5 Antibody

• Catalog Number: orb1247742
• Category: Antibodies
• Description: BAG5 Antibody
• Target: BAG5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: DMGNQHPSISRLQ-C
• UniProt ID: Q9UL15
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55+48kDa bands observed in nuclear lysates of cell line HeLa (calculated MW of 56.0kDa according to NP_001015049.1 and of 51.2kDa according to NP_004864.1; NP_001015048.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BAG5, BCL2-associated athanogene 5, BAG-5, KIAA087
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_004864.1, NP_001015049.1, NP_001015048.1

orb1247742 (2 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247742 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.