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B23 (phospho Thr199) rabbit pAb

Catalog Number: orb769288

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb769288
CategoryAntibodies
DescriptionRabbit polyclonal antibody to B23 (phospho-Thr199)
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
MW32
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-NPM1 antibody, anti-NPM antibody, anti-Nucleo
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NoteFor research use only
B23 (phospho Thr199) rabbit pAb

Western blot analysis of B23 (phospho Thr199) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

B23 (phospho Thr199) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NPM (Phospho-Thr199) Antibody.

B23 (phospho Thr199) rabbit pAb

Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.

B23 (phospho Thr199) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.

  • Phospho-B23 (Thr199) Rabbit pAb [orb2715482]

    ELISA,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • Phospho-NPM (Thr199) Rabbit pAb, IRDye 800CW conjugated [orb2889527]

    WB

    Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Sheep

    Mouse, Rat

    Rabbit

    Polyclonal

    IRDye800

    100 μl