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B23 (phospho Thr199) rabbit pAb

Catalog Number: orb769288

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb769288
CategoryAntibodies
DescriptionRabbit polyclonal antibody to B23 (phospho-Thr199)
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
ConjugationUnconjugated
MW32
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-NPM1 antibody, anti-NPM antibody, anti-Nucleo
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NoteFor research use only
Expiration Date12 months from date of receipt.
B23 (phospho Thr199) rabbit pAb

Western blot analysis of B23 (phospho Thr199) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

B23 (phospho Thr199) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NPM (Phospho-Thr199) Antibody.

B23 (phospho Thr199) rabbit pAb

Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.

B23 (phospho Thr199) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is blocked with the phospho peptide.

  • Phospho-B23 (Thr199) Rabbit pAb [orb2715482]

    ELISA,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl