Azithromycin

SKU: orb1300799

Description

Azithromycin (CP 62993) is a macrolide antibiotic that inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit. It is widely used in research for studying respiratory, urogenital, and skin infections in both in vitro and in vivo experimental models.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 83905-01-5
• MW: 748.98
• Purity: 99.97% (May vary between batches)
• Formula: C₃₈H₇₂N₂O₁₂
• SMILES: O([C@@H]1[C@@H](C)[C@H](O[C@H]2C[C@](OC)(C)[C@@H](O)[C@H](C)O2)[C@@H](C)C(=O)O[C@H](CC)[C@@](C)(O)[C@H](O)[C@@H](C)N(C)C[C@H](C)C[C@@]1(C)O)[C@H]3[C@H](O)[C@@H](N(C)C)C[C@@H](C)O3
• Target: Antibacterial,Autophagy,Parasite,Antibiotic
• Solubility: DMSO:18.33 mg/mL (24.47 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (2.67 mM);Ethanol:74.9 mg/mL (100 mM)

Bioactivity

• Target IC50:
  NF-κB:56 μM (A549 cells)|IL-1β:17.4 μM|Parasite growth:8.4 ± 1.2 μM|TNF-α:10.66 μM|PBMC cells proliferation:5 µg/mL
• In Vivo:
  METHODS: To study the effect of Azithromycin on asthma, a mouse model of asthma attack was treated with Azithromycin (50 mg/kg). RESULTS: Azithromycin had no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin did not induce general inflammatory parameters or LDH release in a mouse model of asthma exacerbations and increased expression of an interferon-stimulated gene and the pattern recognition receptor MDA5 but not RIG-I in mice with asthma exacerbations.
• In Vitro:
  METHODS: HeLa, MG-63 and Osteoblast cells were treated with Azithromycin (0-400 μg/mL) for 48 hours, and MTT assay was used to detect the inhibition of cell metabolic activity. RESULTS: Azithromycin inhibited the growth of HeLa (IC50=160 μM), MG-63 (IC50=180 μM) and Osteoblast (IC50=160 μM) cells.
• Cell Research:
  THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, CP 62993, CP62993, CP-62993, Antibiotic, Azithromycin, Autophagy, Bacterial, Zithromax, protein synthesis, XZ 450, XZ450, XZ-450

Compound Identifiers

PubChem CID

447043