Azithromycin hydrate

SKU: orb1308671

Description

Azithromycin hydrate

Research Area

Cell Biology, Infectious Disease & Virology

Key Properties

• CAS Number: 117772-70-0
• MW: 785.03
• Purity: 99.90%
• Formula: C₃₈H₇₂N₂O₁₂·2H₂O
• SMILES: N1([C@@H]([C@H]([C@@]([C@H](OC(=O)[C@@H]([C@H]([C@@H]([C@H]([C@@](C[C@H](C1)C)(O)C)O[C@H]1[C@@H]([C@H](C[C@H](O1)C)N(C)C)O)C)O[C@H]1C[C@]([C@H]([C@@H](O1)C)O)(OC)C)C)CC)(O)C)O)C)C.O.O
• Target: ribosome,Autophagy,Antibiotic,Parasite,Antibacterial
• Solubility: DMSO:50 mg/mL (63.69 mM);Ethanol:93 mg/mL (118.47 mM);H2O:10 mg/mL (12.74 mM)

Bioactivity

• In Vivo:
  Azithromycin (50 mg/kg) does not alter bronchoalveolar lavage inflammatory parameters or LDH levels in a mouse asthma exacerbation model. It also does not induce general inflammation or LDH release, but enhances interferon-stimulated gene expression and MDA5, a pattern recognition receptor, effectively lowering viral load without affecting RIG-I in exacerbating mice.
• Cell Research:
  THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CP62993, CP-62993, CP62993 Dihydrate, CP-62993 dihydrate, CP-62993 Dihydrate, CP 62993, CP 62993 Dihydrate, 50S ribosome, 23S rRNA, Antibiotic, Autophagy, Azithromycin, Azithromycin Dihydrate, Azithromycin dihydrate, Azithromycin hydrate, Azithromycin Hydrate, Bacterial, inhibit, Inhibitor

Compound Identifiers

PubChem CID

3033819