Azathramycin

SKU: orb1304368

Description

Azathramycin (Desmethyl Azithromycin) is a macrolide antibiotic derived from erythromycin that inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit. It is a valuable research tool for studying macrolide mechanisms and bacterial resistance in both in vitro and in vivo experimental models.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 76801-85-9
• MW: 734.96
• Purity: 99.71%
• Formula: C₃₇H₇₀N₂O₁₂
• SMILES: O([C@H]1[C@H](C)[C@@H](O[C@H]2[C@H](O)[C@@H](N(C)C)C[C@@H](C)O2)[C@](C)(O)C[C@@H](C)CN[C@H](C)[C@@H](O)[C@](C)(O)[C@@H](CC)OC(=O)[C@@H]1C)[C@H]3C[C@](OC)(C)[C@@H](O)[C@H](C)O3
• Target: Antibiotic,Antibacterial
• Solubility: DMSO:7.35 mg/mL (10 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (1.36 mM)

Bioactivity

• Cell Research:
  THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, 6-Demethylazithromycin, Antibiotic, Azaerythromycin, Azathramycin, Bacterial, Azaerythromycin A, Desmethyl Azithromycin

Compound Identifiers

PubChem CID

9810601