Azathioprine

SKU: orb1225029

Description

Azathioprine is an immunosuppressive drug, inhibiting purine synthesis and GTP-binding protein Rac1 activation, used in the treatment of organ transplantation and autoimmune diseases.(In Vitro):Azathioprine (0-50 μM, 48 hours) can induce severe intracellular GSH depletion with relevant concentrations in both primary rat and human hepatocytes.(In Vivo):Azathioprine (oral gavage, 25-400 mg/kg, everyday, 10days) can affect bone marrow cells, red blood cells, and peripheral blood cytokines and other related parameters in a dose-dependent manner, and can induce apoptosis in female CD-1 mice and ICR mice.

Key Properties

• CAS Number: 446-86-6
• MW: 277.26
• Purity: >98% (HPLC)
• Formula: C₉H₇N₇O₂S
• SMILES: O=[N+](C1=C(SC2=C3NC=NC3=NC=N2)N(C)C=N1)[O-]
• Target: GPR
• Solubility: DMSO: 54 mg/mL (194.76 mM)

Bioactivity

• In Vivo:
  Azathioprine (oral gavage, 25-400 mg/kg, everyday, 10days) can affect bone marrow cells, red blood cells, and peripheral blood cytokines and other related parameters in a dose-dependent manner, and can induce apoptosis in female CD-1 mice and ICR mice. Animal model: Outbred female CD-1 mice, Female ICR mice. Dosage: 25-400 mg/kg. Administration: Oral gavage; everyday; 10 days. Result: Induced a decrease in erythrocyte-related parameters as well as leukocyte-related parameters in a dose-dependent manner. Induced 52.4%, 35.4%, 17.9%, 16.1% and 15.2% reduction in bone marrow cells at concentrations of 40, 60, 80, 100 and 120 mg/kg, respectively while fms-like tyrosine kinase-3(FLT-3) ligand (FL)-related cytokines were increased.
• In Vitro:
  Azathioprine (0-50 μM, 48 hours) can induce severe intracellular GSH depletion with relevant concentrations in both primary rat and human hepatocytes. Cell Viability Assay Cell line: Rat hepatocytes, Human hepatocytes. Concentration: 0-50 μM. Incubation time: 24-48 hours. Result: Showed the decrease in cell viability and intracellular GSH levels in rat hepatocytes as low concentration of 0.5 μM but no significant decrease in cell viability at concentrations below 50 μM as well as GSH depletion was obviously noted at a concentration as low as 1 μM in human hepatocytes.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Azamune | Azoran | BW 57-322 | Imuran | NSC 39084

Quick Database Links

Gene Symbol

GPR