Avidin Antibody Peroxidase Conjugated

SKU: orb344922

Description

Avidin antibody (Peroxidase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 1:250 - 1:1,000, WB: 1:1,000 - 1:2,500
• Reactivity: Bacteria
• Application Notes:
  Anti-Avidin Antibody Peroxidase Conjugated has been tested in WB and assayed against 1.0 ug of Avidin [Hen Egg White ] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:27,000 of the reconstitution concentration is suggested for this product. This product can be suitable for ELISA, Western Blot, and Immunohistochemistry.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Avidin (Hen Egg White)
• Purity: Anti-Avidin Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Avidin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Avidin antibody, Avidin Peroxidase, Anti-Avidin HRP Antibody

Western Blot of Rabbit Anti-Avidin Antibody Peroxidase Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Avidin [0.05 µg]. Lane 3: Streptavidin [0.05 µg]. Primary Antibody: Anti-Avidin HRP at 1.0 µg/ml overnight at 2-8°C. Secondary Antibody: none. Blocking Buffer: BlockOut (p/n orb348644) for 30 mins at RT. Predicted MW: ~17kDa.

Quick Database Links

UniProt

P02701