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| Catalog Number | orb1146716 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tartrate Resistant Acid Phosphatase 5b(TRACP-5b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tartrate Resistant Acid Phosphatase 5b(TRACP-5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tartrate Resistant Acid Phosphatase 5b(TRACP-5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tartrate Resistant Acid Phosphatase 5b(TRACP-5b) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Concentration | 50 ng/mL |
| Application notes | standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.78-50 ng/mL |
| Sensitivity | 0.46 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | ACP5,TRAP5b,SPENCDI |
| Note | For research use only |
Mouse ACP5 ELISA Kit [orb409190]
Mouse
0.078 mIU/mL-5 mIU/mL
0.02 mIU/mL
10 x 96 T, 48 T, 24 T, 96 T, 5 x 96 T- Item 1 of 1
Mouse Tartrate Resistant Acid Phosphatase 5b (TRACP-5b) ELISA Kit [orb1807786]
Mouse
0.78-50ng/mL
0.47 ng/mL
96 T, 48 T
