Human ComplementiC3b (iC3b) ELISA Kit

• Catalog Number: orb1146729
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ComplementiC3b(iC3b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ComplementiC3b(iC3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ComplementiC3b(iC3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ComplementiC3b(iC3b) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 200 ng/mL
• Application notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human iC3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human iC3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human iC3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human iC3b in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.88 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: iC3b
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only
• Expiration Date: Please enquire.

References

S Mami, S Shekarchian, AN Sohi, J Kiani, M Soleimani, MH Nicknam In vitro Safety and Immunotoxicity Assessment of a Novel mRNA-LNP Vaccine against Cytomegalovirus: Insights into Safety and Immunomodulatory Profiles Iranian Journal of Allergy, (2025)

PubMed:

Applications

ELISA

Reactivity

Human