Avian Influenza Neuraminidase Antibody

• Catalog Number: orb1238122
• Category: Antibodies
• Description: Avian Influenza Neuraminidase Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Avian influenza neuraminidase antibody was raised against a synthetic peptide corresponding to 16 amino acids in the middle of the avian influenza neuraminidase protein.Efforts were made to use relatively conserved regions as the antigen.The immunogen is located within amino acids 150 - 200 of Avian Influenza Neuraminidase.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NA
• UniProt ID: Q710U6
• NCBI: CAC95053
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Avian Influenza Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Avian Influenza Neuraminidase Antibody: Neuraminid
• Note: For research use only
• Application notes: WB: 1 μg/mL. Avian Influenza Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Avian Influenza NA Protein. Loading: 50 ng (Lane 1) and 100 ng (Lane 2) Avian Influenza Neuraminidase recombinant protein. Antibodies: Avian Influenza antibody, orb1238122, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ELISA Validation with Avian Influenza NA Protein. Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 µg/mL, incubated at 4°C overnight. Detection Antibodies: Avian Influenza NA antibody, orb1238122, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:10000, incubated at RT for 1 hr.