Avian Influenza Neuraminidase Antibody

• Catalog Number: orb1238121
• Category: Antibodies
• Description: Avian Influenza Neuraminidase Antibody
• Target: NA
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: Avian Influenza Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: Avian Influenza Neuraminidase Antibody is affinity chromatography purified via peptide column.
• Immunogen: Avian influenza neuraminidase antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the avian influenza neuraminidase protein.Efforts were made to use relatively conserved regions as the antigen.The immunogen is located within the last 50 amino acids of Avian Influenza Neuraminidase.
• UniProt ID: Q710U6
• Tested applications: ELISA, WB
• Application notes: WB: 1 μg/mL. Avian Influenza Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Avian Influenza Neuraminidase Antibody: Neuraminid
• Note: For research use only
• NCBI: CAC95053

Western Blot Validation with Avian Influenza NA Protein. Loading: 50 ng (Lane 1) and 100 ng (Lane 2) Avian Influenza Neuraminidase recombinant protein. Antibodies: Avian Influenza antibody, orb1238121, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ELISA Validation with Avian Influenza NA Protein. Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 µg/mL, incubated at 4°C overnight. Detection Antibodies: Avian Influenza NA antibody, orb1238121, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:10000, incubated at RT for 1 hr.