Aurora-C Antibody (N-term G11)

SKU: orb1429375

Description

Rabbit polyclonal antibody to Aurora-C (G11).

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution range: WB: 1:1000, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB6824
• Immunogen: Synthetic Peptide
• Target: AURKC
• Molecular Weight: 35591
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, ARK-3, Aurora-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, AURKC, AIE2, AIK3, AIRK3, ARK3, STK13

Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).

The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).

Quick Database Links

Gene Symbol

AURKC

UniProt

Q9UQB9

RefSeq (Protein):NP_001015879.1, NP_001015878.1, NP_003151.2