Aurora-A Antibody (C-term)

SKU: orb1929657

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD8056
• Immunogen: This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A. Antigen Region: 364-392 aa.
• Target: AURKA (HGNC:11393)
• Molecular Weight: 45823 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA

Aurora-A Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of Aurora-A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of anti-Aurora-A Pab in A2058 cell line lysate.Aurora-A (arrow) was detected using the purified Pab.

Western blot analysis of Aurora-A (arrow) using rabbit polyclonal Aurora-A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2).

Quick Database Links

Gene Symbol

AURKA (HGNC:11393)

UniProt

O14965

RefSeq (Protein):NP_940838.1, NP_003591.2, NP_940835.1, NP_940839.1, NP_940837.1, NP_940836.1