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Aurora-A Antibody (C-term)
Description
Images & Validation
−| Tested Applications | FC, IF, WB |
|---|---|
| Dilution range | WB - 1:1000, IF - 1:10-50, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 45809 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Aurora A Antibody [orb224015]
WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 30 μl, 50 μl

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Confocal immunofluorescent analysis of Aurora-A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of anti-Aurora-A Pab in A2058 cell line lysate.Aurora-A (arrow) was detected using the purified Pab.

Western blot analysis of Aurora-A (arrow) using rabbit polyclonal Aurora-A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2).

Aurora-A Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
Aurora-A Antibody (C-term) (orb1929657)
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![Aurora A antibody [C3], C-term](/images/quality_badge_antibodies.png)