ATP6V1G3 Antibody (N-Term)

SKU: orb1925394

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:25, WB - 1:2000, FC - 1:25
• Reactivity: Human, Mouse
• Predicted Reactivity: Other

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB58081
• Immunogen: This ATP6V1G3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the human region of human ATP6V1G3.
• Target: ATP6V1G3
• Molecular Weight: 13917 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

V-type proton ATPase subunit G 3, V-ATPase subunit G 3, V-ATPase 13 kDa subunit 3, Vacuolar proton pump subunit G 3, ATP6V1G3, ATP6G3

All lanes: Anti-ATP6V1G3 Antibody (N-Term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: Caki-1 whole cell lysate. Lane 3: Renca whole cell lysate. Lane 4: Mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling ATP6V1G3 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

ATP6V1G3

UniProt

Q96LB4