ATP6 Rabbit pAb Discontinued

SKU: orb373996

Description

Rabbit polyclonal antibody to ATP6

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 127-226 of mouse ATP6 (NP_904333.1).
• Molecular Weight: 20kDa
• Purity: Affinity purification
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MT-ATP6, MTATP6, ATPASE6

Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb (orb373996) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb (orb373996) at 1:2000；1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Quick Database Links

UniProt

P00848

NCBI Gene ID

17705

Li, Hong-Sheng et al. WITHDRAWN:Mitochondrial targeting of HIF-1α inhibits hypoxia-induced apoptosis independently of its transcriptional activity Free Radic Biol Med, (2018)

PubMed: 29704620