ATP5O Antibody (N-term)

SKU: orb1928432

Description

ATP5O Antibody (N-term)

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Bovine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 23277 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

ATP5O, ATPO

Western blot analysis of ATP5O Antibody (N-term) in 293 cell line lysates (35 ug/lane). ATP5O (arrow) was detected using the purified Pab.

Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ATP5O (arrow) using rabbit polyclonal ATP5O Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.

Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

UniProt

P48047