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ATP5C1 Antibody
SKU: orb1259599
Featured
Description
Research Area
Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1). |
| Target | ATP5C1 |
| Molecular Weight | Observed: 36kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ATP5C1, ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1, ATP5C, ATP5CL1, ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase, gamma subunit 1
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Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (orb1259599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5S.
Immunofluorescence analysis of C6 cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
ATP5C1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ATP5C1 Antibody (orb1259599)
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