ATP5C1 Antibody

• Catalog Number: orb1259599
• Category: Antibodies
• Description: ATP5C1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 36kDa
• Target: ATP5C1
• UniProt ID: P36542
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ATP5C1, ATP synthase, H+ transporting, mitochondri
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (orb1259599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5S.

Immunofluorescence analysis of C6 cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ATP5C1 Polyclonal Antibody (orb1259599) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.