ATP1B2 Antibody (Center)

• Catalog Number: orb1788196
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 115-141 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 33367 Da
• Target: This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
• UniProt ID: P14415
• NCBI: NP_001669.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ATP1B2; Sodium/potassium-transporting ATPase subun
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ATP1B2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from human brain, mouse brain and human cerebellum tissue lysate (from left to right), using ATP1B2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.