ATP1A4 Antibody (N-Term)

SKU: orb1925451

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD79651
• Immunogen: This ATP1A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-46 amino acids from the human region of human ATP1A4. Antigen Region: 12-46 aa.
• Target: ATP1A4 (HGNC:14073)
• Molecular Weight: 114166 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2

Anti-ATP1A4 Antibody (N-Term) at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 114 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

ATP1A4 (HGNC:14073)

UniProt

Q13733