ATP1A2 Antibody (Center)

SKU: orb1930343

Description

ATP1A2 Antibody (Center)

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 112265 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

KIAA0778

ATP1A2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ATP1A2 Antibody (Center) with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ATP1A2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP1A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ATP1A2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (15 ug/lane). This demonstrates the ATP1A2 antibody detected ATP1A2 protein (arrow).

ATP1A2 Antibody (Center) western blot analysis in mouse heart tissue lysates (15 ug/lane). This demonstrates the ATP1A2 antibody detected ATP1A2 protein (arrow).

Anti-ATP1A2 Antibody (Center) at 1:2000 dilution + human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 112 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P50993