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PNPLA2 Antibody

SKU: orb1247752

Description

PNPLA2 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NIIEVSKEARKR
TargetPNPLA2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

ATGL, DESNUTRIN, PNPLA2, patatin-like phospholipase domain containing 2, 1110001C14Rik, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase

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PNPLA2 Antibody

orb1247752 (0.5 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

PNPLA2 Antibody

orb1247752 (2.5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PNPLA2 Antibody (orb1247752)

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0.1 mg
$ 880.00
DispatchUsually dispatched within 5-10 working days
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