ATG9B Antibody

SKU: orb1240176

Description

ATG9B Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B.The immunogen is located within amino acids 850 - 900 of ATG9B.
• Target: ATG9B
• Purification: ATG9B Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ATG9B Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

ATG9B Antibody: SONE, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Protein sONE

Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 µg/mL.

Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 µg/mL.

WB Validation in Human Tonsil Loading: 15 µg of human tonsil lysate. Antibodies: ATG9B orb1240176, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Quick Database Links

Gene Symbol

ATG9B

UniProt

Q674R7

RefSeq (Protein):NP_775952