ATG9A Antibody

SKU: orb1933422

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB13941
• Immunogen: This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~753 amino acids surrounding S738 of human APG9L1. Antigen Region: 723-753 aa.
• Target: ATG9A {ECO:0000303|PubMed:20124090, ECO:0000312|HGNC:HGNC:22408}
• Molecular Weight: 94447 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy-related protein 9A, APG9-like 1, mATG9, ATG9A, APG9L1

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG9L1 Antibody (S738), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-APG9L1 Antibody (S738) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 94kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ATG9A {ECO:0000303|PubMed:20124090, ECO:0000312|HGNC:HGNC:22408}

UniProt

Q7Z3C6

RefSeq (Protein):NP_076990.4, NP_001070666.1