ATG4B Antibody: HRP

• Catalog Number: orb383939
• Category: Antibodies
• Description: Rabbit polyclonal antibody against ATG4B conjugated to HRP.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: HRP
• MW: 44 kDa
• Target: ATG4B
• Entrez: 23192
• UniProt ID: Q9Y4P1
• NCBI: NP_037457.3
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: MGC1353 antibody, Autophagy-related cysteine endop
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.

Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band (s): Band appears at ~40 kDa.