ATF7 Antibody

• Catalog Number: orb1247753
• Category: Antibodies
• Description: ATF7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HVIMTPQSQSAGR
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ATF7
• UniProt ID: P17544
• NCBI: NP_001123532.1, NP_006847.1, NP_001123531.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ATF7, ATFA, activating transcription factor 7, MGC
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2ug/ml. Please note the band size is in agreement with previous experimental data (J Biol Chem 2001 Apr 27;276(17):13718-26).Immunohistochemistry:In paraffin embedded Human Testis shows staining of select spermatocytes in Human Testis. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247753 staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247753 (3.8 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.