ATF6 Antibody

• Catalog Number: orb248140
• Category: Antibodies
• Description: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: 1X PBS, pH 7.4
• Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 2-4ug/ml (2),IHC (Paraffin): 5ug/ml (1)
• Application notes: ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Antibody Type: Primary Antibody
• Clone Number: AF6229
• Formula: 0.5 mg/ml antibody in PBS with 0.1 mg/ml BSA (US source) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

References

Merna Roshdy 1, Doaa A Zaky 2, Samah S Abbas 1, Dalaal M Abdallah Niacin, an innovative protein kinase-C-dependent endoplasmic reticulum stress reticence in murine Parkinson's disease Life Sci, (2024)

PubMed: 38914304

Applications

WB

Reactivity

Rat

Western blot testing of human samples with ATF6 antibody (AF6229) at 2 ug/ml. Predicted molecular weight: 75~90kDa.

IHC testing of FFPE human breast tissue with ATF6 antibody at 5 ug/ml.