ATF6 Antibody-

SKU: orb248140

Description

ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 2-4ug/ml (2),Immunohistochemistry (FFPE): 5ug/ml (1)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: AF6229
• Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
• Purification: Protein G purified monoclonal antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Merna Roshdy 1, Doaa A Zaky 2, Samah S Abbas 1, Dalaal M Abdallah Niacin, an innovative protein kinase-C-dependent endoplasmic reticulum stress reticence in murine Parkinson's disease Life Sci, (2024)

PubMed: 38914304

Applications

WB

Reactivity

Rat