ATF6 Antibody

• Catalog Number: orb248140
• Category: Antibodies
• Description: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AF6229
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-4ug/ml (2),IHC (Paraffin): 5ug/ml (1)
• Purity: Protein G purified monoclonal antibody
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml antibody in PBS with 0.1 mg/ml BSA (US source) and 0.05% sodium azide
• Hazard Information: This ATF6 antibody is available for research use only.
• Entrez: 22926
• Storage: Aliquot the antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast tissue using ATF6 antibody

Western blot analysis of human samples using ATF6 antibody