ATF6 Antibody

• Catalog Number: orb1239224
• Category: Antibodies
• Description: ATF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ATF6
• UniProt ID: P18850
• NCBI: NP_031374
• Storage: ATF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcr
• Note: For research use only
• Application notes: WB: 0.5-1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

KO Validation of ATF6. Loading: 15 µg of human HeLa cell lysate Antibodies ATF6 orb1239224, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Human Cell Lines. Loading: 15 µg of human cell lysate Antibodies ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.

WB Validation in Mouse Tissue. Loading: 15 µg of mouse tissue lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Heart. Loading: 15 µg of rat kidney lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of ATF6 In A549 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ATF6 with orb1239224 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ATF6 in Human Testis Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis labeling ATF6 with orb1239224 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of ATF6 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATF6 antibody (orb1239224) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ATF6 in Rat Brain Tissue. Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (orb1239224) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.