ATF6 Antibody

• Catalog Number: orb1239224
• Category: Antibodies
• Description: ATF6 Antibody
• Target: ATF6
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: ATF6 Antibody is affinity chromatography purified via peptide column.
• Immunogen: ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6.
• UniProt ID: P18850
• Tested applications: ELISA, IHC-P, WB
• Application notes: WB: 0.5-2 μg/mL; IHC: 5 μg/mLAntibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcr
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_031374
• Expiration Date: 12 months from date of receipt.

KO Validation of ATF6, Loading: 15 µg of human HeLa cell lysate Antibodies ATF6 orb1239224, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Human Cell Lines, Loading: 15 µg of human cell lysate Antibodies ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.

WB Validation in Mouse Tissue, Loading: 15 µg of mouse tissue lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Heart, Loading: 15 µg of rat kidney lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of ATF6 in Human Testis Tissue, Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue, Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue, Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.