ATF6 Antibody

• Catalog Number: orb1239223
• Category: Antibodies
• Description: ATF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.The immunogen is located within amino acids 470 - 520 of ATF6.
• Concentration: 1 mg/mL
• Dilution range: ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ATF6
• UniProt ID: P18850
• NCBI: NP_031374
• Storage: ATF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcr
• Note: For research use only
• Application notes: ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 µg/mL.

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 µg/mL.

Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 µg/ml.

Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.

Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 µg/ml.

Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.