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Ataxin 1 Antibody: RPE
Catalog Number: orb151106
| Catalog Number | orb151106 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Ataxin 1 (RPE). Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N65/37 (Formerly sold as S65-37) |
| Tested applications | ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Synthetic peptide amino acids 746-761 (RKRRWSAPETRKLEKS) of mouse ataxin-1. Rat: 93% identity (15/16 amino acids identical). Human: 87% identity (14/16 amino acids identical). |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | RPE |
| MW | 85kDa |
| Target | Ataxin 1 |
| Entrez | 20238 |
| UniProt ID | P54254 |
| NCBI | NP_001186233.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | Ataxin-1 antibody, ATX1 antibody, Atxn1 antibody, Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-475 was sufficient for detection of Ataxin 1 in 20 µg of COS cells transiently transfected with Ataxin-1 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone N65/37. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ataxin 1 Antibody (D) Composite.
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