ASRGL1 Antibody

SKU: orb750018

Description

ASRGL1 (Asparaginase-like protein 1), also known as CRASH, belongs to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ASRGL1 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CRASH/1289
• Immunogen: A human partial recombinant protein was used as the immunogen for this ASRGL1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human fallopian tube with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human uterus with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free ASRGL1 Antibody (clone CRASH/1289). Confirmation of Integrity and Purity of the Antibody.