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Catalog Number | orb1256361 |
---|---|
Category | Antibodies |
Description | ASPSCR1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 80kDa |
Target | ASPSCR1 |
UniProt ID | Q9BZE9 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Tether containing UBX domain for GLUT4, Alveolar s Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (orb1256361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (orb1256361). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (orb1256361) at a dilution of 1:1000.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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