ASPA Antibody

SKU: orb1263617

Description

ASPA Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human ASPA.
• Target: ASPA
• Molecular Weight: 36 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Aspartoacylase, Aminoacylase-2, ACY-2, ASPA, ACY2, ASP

Western blot analysis of ASPA Antibody in MCF7 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ASPA using rabbit polyclonal ASPA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.

Confocal immunofluorescent analysis of ASPA Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

ASPA

UniProt

P45381

RefSeq:P45381