ASMT Antibody (Center)

• Catalog Number: orb1930816
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23845
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:500-1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 38453 Da
• Target: This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT.
• UniProt ID: P46597
• NCBI: NP_001164510.1, NP_004034.2, NP_001164509.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Acetylserotonin O-methyltransferase, Hydroxyindole
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ASMT Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ASMT Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of ASMT Antibody (Center) in 293 cell line lysates (35 ug/lane). ASMT (arrow) was detected using the purified Pab.

Western blot analysis of lysate from Y79 cell line, using ASMT Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.