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Catalog Number | orb1248098 |
---|---|
Category | Antibodies |
Description | ASIC1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Porcine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-NILPHHPARGT |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Mouse fetal Brain lysates (calculated MW of 64.8kDa according to NP_064423.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Pancreas. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 70kDa |
Target | ASIC1 |
UniProt ID | P78348 |
NCBI | NP_001243759.1, NP_064423.2, NP_001086.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | ASIC1, acid-sensing (proton-gated) ion channel 1, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Mouse fetal Brain lysates (calculated MW of 64.8kDa according to NP_064423.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Pancreas. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248098 (0.3 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248098 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1248098 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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