ASIC1 Antibody (HRP)

SKU: orb150288

Description

Mouse monoclonal to ASIC1 (HRP). Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4. 3-kb transcript with localization to rat dorsal root ganglia. In situ hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety..

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N271/44 (Formerly sold as S271-44)
• Immunogen: Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
• Target: ASIC1
• Molecular Weight: 60kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASIC1, ACCN2, BNAC2, Acid-sensing ion channel 1, Acid-sensing (proton-gated) ion channel, Amiloride-sensitive cation channel 2 neuronal, Brain sodium channel 2

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ASIC1 Monoclonal Antibody, Clone N271/44. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ASIC1 Antibody (D) Composite.

Western Blot analysis of Rat brain lysates showing detection of ASIC1 protein using Mouse Anti-ASIC1 Monoclonal Antibody, Clone N271/44. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.

Quick Database Links

Gene Symbol

ASIC1

UniProt

Q6NXK8

NCBI Gene ID

11419

RefSeq (Protein):NP_033727.1