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Ascorbate Oxidase Antibody Peroxidase Conjugated
Catalog Number: orb344877
| Catalog Number | orb344877 |
|---|---|
| Category | Antibodies |
| Description | Ascorbate Oxidase antibody (Peroxidase) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | HRP |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Purity | Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources is unknown. |
| Immunogen | Ascorbate Oxidase [cucurbita] |
| UniProt ID | P24792 |
| Tested applications | ELISA, IP, WB |
| Dilution range | ELISA: 1:3,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000 |
| Application notes | Anti-Ascorbate Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-Ascorbate Oxidase Antibody Peroxidase Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
