ASCL4 Antibody

• Catalog Number: orb1259949
• Category: Antibodies
• Description: ASCL4 Antibody
• Target: ASCL4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human ASCL4 (NP_982260.2).
• UniProt ID: Q6XD76
• MW: Observed: 19kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ASCL4, HASH4, bHLHa44
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ASCL4 antibody (orb1259949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.