ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220)

• Catalog Number: orb1939527
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB10320
• Tested applications: WB
• Predicted Reactivity: Mouse, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 25454 Da
• Target: This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ASCL1.
• UniProt ID: P50553
• NCBI: NP_004307.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Achaete-scute homolog 1, ASH-1, hASH1, Class A bas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Ascl1 (Human C-term) at 1:1000 dilution. Lane 1: human brain lysate. Lane 2: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35 ug/lane). Ascl1 (arrow) was detected using the purified Pab (1:160 dilution).

Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2).