PYCARD Antibody

SKU: orb1258912

Description

PYCARD Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein of human ASC / TMS1
• Target: PYCARD
• Molecular Weight: Observed: 25kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC

Western blot analysis of extracts of various cell lines, using ASC/TMS1 antibody (orb1258912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human placenta using ASC/TMS1 antibody (orb1258912) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2 OS cells using ASC/TMS1 Polyclonal Antibody (orb1258912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of U937 cells using 3 ug ASC/TMS1 antibody (orb1258912). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (orb1258912) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

PYCARD

UniProt

Q9ULZ3