ASAH1 Antibody

• Catalog Number: orb1239216
• Category: Antibodies
• Description: ASAH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1.The immunogen is located within amino acids 240 - 290 of ASAH1.
• Concentration: 1 mg/mL
• Dilution range: ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 45 kDaObserved: 49 kDa
• Target: ASAH1
• UniProt ID: Q13510
• NCBI: EAW63795
• Storage: ASAH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ASAH1 Antibody: AC, PHP, ASAH, PHP32, ACDase, SMAP
• Note: For research use only
• Application notes: ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1 µg/mL.

Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 µg/mL.

Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 µg/mL.

Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5 µg/mL.

Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 µg/mL. Red: ASAH1 Antibody (orb1239216) Blue: DAPI staining.