Artesunate

SKU: orb1310621

Description

Artesunate (WR-256283) is a water-soluble, semi-synthetic artemisinin derivative used as a key antimalarial agent in research. Its application extends to studying mechanisms of action and drug resistance in both in vitro parasite assays and in vivo animal models of malaria.

Research Area

Cell Biology, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 88495-63-0
• MW: 384.42
• Purity: 99.91% (May vary between batches)
• Formula: C₁₉H₂₈O₈
• SMILES: C[C@@H]1[C@]2([C@]34[C@](O[C@H]1OC(CCC(O)=O)=O)(O[C@](C)(OO3)CC[C@]4([C@H](C)CC2)[H])[H])[H]
• Target: Ferroptosis,Virus Protease,Parasite,STAT
• Solubility: DMSO:250 mg/mL (650.33 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (13.01 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:9 mg/mL (23.41 mM)

Bioactivity

• In Vivo:
  Artesunate is a medication used to treat malaria. Artesunate can induce radiosensitivity of HeLa cells in vivo using Xenograft model of nude mice. Artesunate had a relatively high immunosuppressive activity with low toxicity, and could inhibit T lymphocyte proliferation induced by mitogen and alloantigen.
• In Vitro:
  ART could alter the biomechanical properties of the glioma cells to inhibit cell proliferation, migration and invasion . The ART significantly suppresses the cell proliferation, induces the apoptosis and promoted cell cycle arrest at G0/G1 phase in SKM-1 cells. The mechanisms of ART anti-MDS is associated with the increase of intracellular calciumion concentration and ROS levels. In addition, the pro-apoptotic activity of ART may be involved in the regulation of BCL-2 /BAX ratio and the expressions of P-bad and survivin. ART treatment demethylates CDH1, which, in turn recovers the E-cadherin activation in SKM-1 cells.
• Cell Research:
  Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Parasite, Ferroptosis, Inhibitor, Artesunate, STAT, WR256283, WR-256283, WR 256283, Virus Protease, VirusProtease

Compound Identifiers

PubChem CID

6917864